Riboswitches : methods and protocols /

Riboswitches : methods and protocols /

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The revolutionary discoveries of RNA interference and bacterial regulatory RNAs led to further breakthroughs such as the identification of riboswitches and related RNA sensors, mRNA regions capable of alternating their conformations in response to the presence of cellular metabolites and other physi...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Serganov, Alexander (Editor)
Format: Book
Language:English
Published: New York : Humana, ©2009
New York : Humana Press : Imprint: Humana Press, c2009
Totowa, NJ : 2009
Series:Methods in Molecular Biology, Methods and Protocols, 540
Methods in molecular biology (Clifton, N.J.) ; v. 540
Methods in molecular biology (Clifton, N.J.), v. 540
Springer Protocols (Springer-12345)
Springer protocols (Series)
Springer protocols
Subjects:
Biochemistry
Gene expression
Life sciences
Ligands (Biochemistry)
Messenger RNA
Nucleic acids
RNA
RNA-protein interactions
RNA > chemistry > Laboratory Manuals
RNA > chemistry
Life Sciences
Gene Expression Regulation > physiology > Laboratory Manuals
Gene Expression Regulation > physiology
Laboratory Techniques and Procedures > Laboratory Manuals
Laboratory Techniques and Procedures
Biochemistry, general
Gene Expression
Nucleic Acid Chemistry
bacteria
biological techniques
biologische technieken
gene regulation
genen
genes
genregulatie
messenger rna
protocollen
protocols
transcriptie
transcription
Moleculaire biologie (algemeen)
Molecular Biology (General)
Laboratory Manual
Electronic books
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245 0 0 |a Riboswitches :  |b methods and protocols /  |c edited by Alexander Serganov 
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490 1 |a Methods in Molecular Biology, Methods and Protocols,  |x 1064-3745 ;  |v 540 
490 1 |a Methods in molecular biology,  |x 1064-3745 ;  |v 540 
490 1 |a Methods in molecular biology,  |x 1064-3745 ;  |v v. 540 
490 1 |a Springer protocols 
500 |a Description based on print version record 
504 |a Includes bibliographical references and index 
505 0 |9 ^^  |a Crystallization of the glmS ribozyme-riboswitch / D.J. Klein and A.R. Ferre-D'Amare -- Riboswitch conformations revealed by small-angle X-ray scattering / J. Lipfert, D. Herschlag and S. Doniach -- Time-resolved NMR spectroscopy: ligand-induced refolding of riboswitches / J. Buck ... [et al.] -- Analysis of the RNA backbone: structural analysis of riboswitches by in-line probing and selective 2'-hydroxyl acylation and primer extension / C.A. Wakeman and W.C. Winkler -- Identification of metabolite-riboswitch interactions using nucleotide analog interference mapping and suppression / J.K. Soukup and G.A. Soukup -- RNA-dependent RNA switches in bacteria / T.M. Henkin -- Probing mRNA structure and sRNA-mRNA interactions in bacteria using enzymes and lead(II) / C. Chevalier ... [et al.] -- Structural probing of RNA thermosensors / C. Chiaruttini, F. Allem and M. Springer --^ 
505 0 |9 ^^  |a Ribosomal initiation complexes probed by toeprinting and effect of trans-acting translational regulators in bacteria / P. Fechter ... [et al.] -- Isolation and characterization of the heat shock RNA 1 / I. Shamovsky and E. Nudler -- Analysis of tRNA-directed transcription antitermination in the T box system in vivo / T.M. Henkin -- In vitro selection of conformational probes for riboswitches / G. Mayer and M. Famulok -- A green fluorescent protein (GFP)-based plasmid system to study post-transcriptional control of gene expression in vivo / J.H. Urban and J. Vogel -- High-throughput screens to discover synthetic riboswitches / S.A. Lynch, S. Topp and J.P. Gallivan -- A mammalian cell-based assay for screening inhibitors of RNA cleavage / L. Yen, B.R. Stockwell and R.C. Mulligan -- In vitro selection of glmS ribozymes / K.H. Link and R.R. Breaker 
505 0 |a Predicting Riboswitch Regulation on a Genomic Scale -- Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemical Modifications -- Application of Fluorescent Measurements for Characterization of Riboswitch-Ligand Interactions -- Transcriptional Approaches to Riboswitch Studies -- Kinetics of Riboswitch Regulation Studied By In Vitro Transcription -- Molecular Basis of RNA-Mediated Gene Regulation on the Adenine Riboswitch by Single-Molecule Approaches -- Methods for Analysis of Ligand-Induced RNA Conformational Changes -- Monitoring RNA-Ligand Interactions Using Isothermal Titration Calorimetry -- Preparation and Crystallization of Riboswitch-Ligand Complexes -- Crystallization of the glmS Ribozyme-Riboswitch -- Riboswitch Conformations Revealed by Small-Angle X-Ray Scattering -- Time-Resolved NMR Spectroscopy: Ligand-Induced Refolding of Riboswitches -- Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2'-Hydroxyl Acylation and Primer Extension -- Identification of Metabolite-Riboswitch Interactions Using Nucleotide Analog Interference Mapping and Suppression -- RNA-Dependent RNA Switches in Bacteria -- Probing mRNA Structure and sRNA-mRNA Interactions in Bacteria Using Enzymes and Lead(II) -- Structural Probing of RNA Thermosensors -- Ribosomal Initiation Complexes Probed by Toeprinting and Effect of trans-Acting Translational Regulators in Bacteria -- Isolation and Characterization of the Heat Shock RNA 1 -- Analysis of tRNA-Directed Transcription Antitermination in the T Box System In Vivo -- In Vitro Selection of Conformational Probes for Riboswitches -- A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo -- High-Throughput Screens to Discover Synthetic Riboswitches -- A Mammalian Cell-Based Assay for Screening Inhibitors of RNA Cleavage -- In Vitro Selection of glmS Ribozymes 
505 0 |a Predicting riboswitch regulation on a genomic scale / J.E. Barrick -- Enzymatic ligation strategies for the preparation of purine riboswitches with site-specific chemical modifications / R. Rieder, C. Hobartner and R. Micura -- Application of fluorescent measurements for characterization of riboswitch-ligand interactions / B. Heppell ... [et al.] -- Transcriptional approaches to riboswitch studies / A. Mironov, V. Epshtein and E. Nudler -- Kinetics of riboswitch regulation studied by in vitro transcription / J.K. Wickiser -- Molecular basis of RNA-mediated gene regulation on the adenine riboswitch by single-molecule approaches / J.F. Lemay ... [et al.] -- Methods for analysis of ligand-induced RNA conformational changes / C.A. Brautigam, C.A. Wakeman and W.C. Winkler -- Monitoring RNA-ligand interactions using isothermal titration calorimetry / S.D. Gilbert and R.T. Batey -- Preparation and crystallization of riboswitch-ligand complexes / O. Pikovskaya ... [et al.] --^ 
505 0 |a Predicting riboswitch regulation on a genomic scale / Jeffrey E. Barrick -- Enzymatic ligation strategies for the preparation of purine riboswitches with site-specific chemical modification / Renate Rieder, Claudia Höbartner and Ronald Micura -- Application of fluorescent measurements for characterization of riboswitch-ligand interactions / Benoit Heppell [and others] -- Transcriptional approaches to riboswitch studies / Alexander Mironov, Vitaly Epshtein and Evgeny Nudler -- Kinetics of riboswitch regulation studied by in vitro transciption / J. Kenneth Wickiser -- Molecular basis of RNA-mediated gene regulation on the adenine riboswitch by single-molecule approaches / Jean-Franc̜ois Lemay [and others] -- Methods for analysis of ligand-induced RNA confromational changes / Chad A. Brautigam, Catherine A. Wakeman and Wade C. Winkler -- Preparation and crystallization of riboswitch-ligand complexes / Olga Pikovskaya [and others] -- Crystallization of the glmS ribozyme-riboswitch / Daniel J. Klein and Adrian R. Ferré-D'Amaré -- Riboswitch conformations revealed by small-angle X-ray scattering / Jan Lipfert, Daniel Herschlag and Sebastian Doniach -- Time-resolved NMR spectroscopy : ligand-induced refolding of riboswitches / Janina Buck [and others] -- Analysis of the RNA backbone : structural Analysis of riboswitches by in-line probing and selective 2'-hydroxyl acylation and primer extension / Catherine A. Wakeman and Wade C. Winkler -- Identification of metabolite-riboswitch interactions using nucleotide analog interference mapping and suppression / Juliane K. Soukup and Garrett A. Soukup -- RNA-dependent RNA switches in bacteria / Tina M. Henkin -- Probing mRNA structure and sRNA-mRNA interactions in bacteria using enzymes and lead(II) / Clément Chevalier [and others] -- Structural probing of RNA thermosensors / Claude Chiaruttini, Frédéric Allem and Mathias Springer -- Ribosomal initiation complexes probed by toeprinting and effect of trans-acting translational regulators in bacteria / Pierre Fechter [and others] -- Isolation and characterization of the heat shock RNA 1 / Ilya Shamovsky and Evgeny Nudler -- Analysis of tRNA-directed transcription antitermination in the T box system in vivo / Tina M. Henkin -- In vitro selection of conformational probes for riboswitches / Günter Mayer and Michael Famulok -- A green fluorescent protein (GFP)-based plasmid system to study post-transcriptional control of gene expression in vivo / Johannes H. Urban and Jörg Vogel -- High-throughput screens to Discover synthetic riboswitches / Sean A. Lynch, Shana Topp and Justin P. Gallivan -- A mammalian cell-based assay for screening inhibitors of RNA cleavage / Laising Yen, Brent R. Stockwell and Richard C. Mulligan -- In vitro selection of glmS ribozymes / Kristian H. Link and Ronald R. Breaker 
506 |a License restrictions may limit access 
506 |a Restricted for use by site license 
520 |a The revolutionary discoveries of RNA interference and bacterial regulatory RNAs led to further breakthroughs such as the identification of riboswitches and related RNA sensors, mRNA regions capable of alternating their conformations in response to the presence of cellular metabolites and other physical or chemical cues. In Riboswitches: Methods and Protocols, expert researchers provide comprehensive and up-to-date coverage of various methods used to study riboswitches and other RNAs involved in gene expression control. Examining biochemical and modern biophysical techniques, the volume focuses on mRNAs responding to small organic molecules but expands the definition of a riboswitch to incorporate classes of RNA that undergo conformational transitions in response to other stimuli in order to control the expression of genes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Riboswitches: Methods and Protocols promises to inspire both novices and experts working in a wide range of contemporary biological areas who wish to further develop their RNA methodology 
520 |a The revolutionary discoveries of RNA interference and bacterial regulatory RNAs led to further breakthroughs such as the identification of riboswitches and related RNA sensors, mRNA regions capable of alternating their conformations in response to the presence of cellular metabolites and other physical or chemical cues. In Riboswitches: Methods and Protocols, expert researchers provide comprehensive and up-to-date coverage of various methods used to study riboswitches and other RNAs involved in gene expression control. Examining biochemical and modern biophysical techniques, the volume focuses on mRNAs responding to small organic molecules but expands the definition of a riboswitch to incorporate classes of RNA that undergo conformational transitions in response to other stimuli in order to control the expression of genes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Riboswitches: Methods and Protocols promises to inspire both novices and experts working in a wide range of contemporary biological areas who wish to further develop their RNA methodology 
546 |a English 
588 |a Description based on print version record 
588 0 |a Print version record 
650 0 |a Biochemistry 
650 0 |a Gene expression 
650 0 |a Life sciences 
650 0 |a Ligands (Biochemistry) 
650 0 |a Messenger RNA 
650 0 |a Nucleic acids 
650 0 |a RNA 
650 0 |a RNA-protein interactions 
650 7 |a Biochemistry  |2 fast 
650 7 |a Gene expression  |2 fast 
650 7 |a Life sciences  |2 fast 
650 7 |a Ligands (Biochemistry)  |2 fast 
650 7 |a Messenger RNA  |2 fast 
650 7 |a Nucleic acids  |2 fast 
650 7 |a RNA  |2 fast 
650 7 |a RNA-protein interactions  |2 fast 
650 1 2 |a RNA  |x chemistry  |v Laboratory Manuals 
650 1 2 |a RNA  |x chemistry 
650 1 4 |a Life Sciences 
650 2 2 |a Gene Expression Regulation  |x physiology  |v Laboratory Manuals 
650 2 2 |a Gene Expression Regulation  |x physiology 
650 2 2 |a Laboratory Techniques and Procedures  |v Laboratory Manuals 
650 2 2 |a Laboratory Techniques and Procedures 
650 2 4 |a Biochemistry, general 
650 2 4 |a Gene Expression 
650 2 4 |a Nucleic Acid Chemistry 
653 0 0 |a bacteria 
653 0 0 |a biological techniques 
653 0 0 |a biologische technieken 
653 0 0 |a gene regulation 
653 0 0 |a genen 
653 0 0 |a genes 
653 0 0 |a genregulatie 
653 0 0 |a messenger rna 
653 0 0 |a protocollen 
653 0 0 |a protocols 
653 0 0 |a transcriptie 
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653 1 0 |a Moleculaire biologie (algemeen) 
653 1 0 |a Molecular Biology (General) 
655 2 |a Laboratory Manual 
655 4 |a Electronic books 
700 1 |a Serganov, Alexander 
700 1 |a Serganov, Alexander,  |e editor 
710 2 |a SpringerLink (Online service) 
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776 0 8 |i Printed edition:  |z 9781934115886 
830 0 |a Methods in Molecular Biology, Methods and Protocols,  |x 1064-3745 ;  |v 540 
830 0 |a Methods in molecular biology (Clifton, N.J.) ;  |v v. 540 
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830 0 |a Springer protocols (Series) 
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