Testing for genetic manipulation in plants /
The debate over genetic manipulation and its use in plant improvement and protection has led to an increased demand for developing methods for detecting and characterizing genetic manipulation in plants and plant products such as seeds and foods. This book is unique in presenting all relevant method...
Other Authors: | , , |
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Format: | Book |
Language: | English |
Published: |
Berlin ; New York :
Springer,
[2002], ©2002
Berlin ; New York : Springer-Verlag Berlin Heidelberg, c2002 New York : 2002 Berlin ; New York : [2002] |
Series: | Molecular methods of plant analysis ;
v. 22 Molecular methods of plant analysis ; v. 22 |
Subjects: |
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245 | 0 | 0 | |a Testing for genetic manipulation in plants / |c edited by J.F. Jackson, H.F. Linskens, and R.B. Inman |
260 | |a Berlin ; |a New York : |b Springer, |c [2002], ©2002 | ||
260 | |a Berlin ; |a New York : |b Springer, |c c2002 | ||
260 | |a New York : |b Springer-Verlag Berlin Heidelberg, |c 2002 | ||
264 | 1 | |a Berlin ; |a New York : |b Springer, |c [2002] | |
264 | 4 | |c ©2002 | |
300 | |a 194 p. ; |c 24 cm | ||
300 | |a xvii, 194 p. : |b 33 fig. (2 col.), 16 tab. ; |c 25 cm | ||
300 | |a xvii, 194 p. : |b ill. ; |c 24 cm | ||
300 | |a xvii, 194 pages : |b illustrations ; |c 24 cm | ||
336 | |a text |b txt |2 rdacontent | ||
337 | |a unmediated |b n |2 rdamedia | ||
338 | |a volume |b nc |2 rdacarrier | ||
440 | 0 | |a Molecular methods of plant analysis ; |v v. 22 | |
490 | 1 | |a Molecular methods of plant analysis ; |v v. 22 | |
500 | |a This WorldCat-derived record is shareable under Open Data Commons ODC-BY, with attribution to OCLC |5 CTY | ||
504 | |a Includes bibliographical references (p. ) | ||
504 | |a Includes bibliographical references (p. ) | ||
504 | |a Includes bibliographical references and index | ||
505 | 0 | 0 | |g 1 |t Selectable and Screenable Markers for Rice Transformation / |r R.M. Twyman, E. Stoger, A. Kohli, T. Capell, P. Christou -- |g 1.2 |t Dominant Selectable Markers for Rice |g 3 -- |g 1.2.1 |t Aminoglycoside 3'-Phosphotransferase (Neomycin Phosphotransferase) |g 3 -- |g 1.2.2 |t Hygromycin Phosphotransferase |g 4 -- |g 1.2.3 |t Phosphinothricin Acetyltransferase |g 4 -- |g 1.2.4 |t Other Dominant Selectable Markers |g 5 -- |g 1.3 |t Novel Selectable Markers |g 7 -- |g 1.3.1 |t Innocuous Markers |g 7 -- |g 1.3.2 |t Counterselectable Markers |g 7 -- |g 1.4 |t Screenable Marker Genes |g 8 -- |g 1.4.1 |t [beta]-Glucuronidase (gusA) |g 9 -- |g 1.4.2 |t Firefly Luciferase (luc) |g 11 -- |g 1.4.3 |t Green Fluorescent Protein (gfp) |g 11 -- |g 1.5 |t Strategies for Marker-Gene Delivery |g 13 -- |g 2 |t Use of Green Fluorescent Protein to Detect Transformed Shoots / |r J. Molinier, G. Hahne -- |g 2.2 |t GFP: Suitable as a Visually Selectable Marker In Planta? |g 20 -- |g 2.2.1 |t Important Properties of the Protein |g 20 -- |g 2.2.2 |t Properties of a Useful Selectable Marker in Plant Transformation Technology |g 21 -- |g 2.3 |t GFP Expression and Detection in Primary Transformed Tissues |g 22 -- |g 2.3.1 |t Transient Expression and GFP Detection |g 22 -- |g 2.3.2 |t Detection Equipment and Troubleshooting |g 22 -- |g 2.3.3 |t Stable Expression and GFP Detection in Primary Transformed Tissues |g 23 -- |g 2.4 |t GFP for Screening of Segregating Populations |g 28 -- |g 3 |t Luciferase Gene Expressed in Plants, Not in Agrobacterium / |r S.L. Mankin -- |g 3.2 |t Preventing Bacterial Expression |g 31 -- |g 3.3 |t Imaging Luciferase Activity In Planta |g 32 -- |g 3.4 |t Measuring Luciferase Activity in Plant Extracts |g 34 -- |g 4 |t Use of [beta]-Glucuronidase (GUS) To Show Dehydration and High-Salt Gene Expression / |r K. Nakashima, K. Yamaguchi-Shinozaki -- |g 4.2 |t What Is GUS? |g 39 -- |g 4.3 |t Trasgenic Plants Carrying Promoter-GUS Constructs |g 41 -- |g 4.3.1 |t Construction of Promoter-GUS Fusion Genes |g 44 -- |g 4.3.2 |t Introduction of Promoter-GUS Constructs into Agrobacterium |g 45 -- |g 4.3.3 |t Transformation of Plants with Agrobacterium |g 46 -- |g 4.3.3.1 |t Transformation of Arabidopsis Plants |g 46 -- |g 4.4 |t Fluorometric Assay |g 49 -- |g 4.4.2 |t Stress Conditions |g 49 -- |g 4.4.2.1 |t Plant Preparation |g 49 -- |g 4.4.2.2 |t Dehydration |g 49 -- |g 4.4.2.3 |t High Salinity |g 49 -- |g 4.4.2.4 |t ABA Treatment |g 50 -- |g 4.4.2.5 |t Other Treatments |g 50 -- |g 4.4.3 |t Protein Assay |g 50 -- |g 4.4.4 |t Sample Preparation |g 50 -- |g 4.4.5 |t Fluorometric Assay |g 51 -- |g 4.5 |t Histochemistry |g 51 -- |g 4.5.2 |t Histochemistry |g 52 -- |g 4.6 |t Northern Analysis of GUS |g 52 -- |g 4.6.2 |t RNA Extraction |g 53 -- |g 4.6.3 |t RNA Blotting |g 54 -- |g 4.6.4 |t Northern Hybridization |g 55 -- |g 4.7 |t Application of the GUS System |g 56 -- |g 4.7.1 |t Transient Assay |g 56 -- |g 4.7.2 |t Transactivation Experiment |g 57 -- |g 4.7.3 |t Promoter Tagging (Enhancer Trap) |g 57 -- |g 5 |t Methods for Detecting Genetic Manipulation in Grain Legumes / |r H.-J. Jacobsen, R. Greiner -- |g 5.2 |t Detection at the DNA Level |g 64 -- |g 5.3 |t PCR Analysis |g 65 -- |g 5.4 |t Control PCR and Specific PCR Systems |g 66 -- |g 5.5 |t Quantitative Approach |g 69 -- |g 5.6 |t Competitive PCR |g 69 -- |g 5.7 |t Real-Time PCR Systems |g 70 -- |g 6 |t Elimination of Selectable Marker Genes from Transgenic Crops / |r A.P. Gleave -- |g 6.2 |t Co-transformation |g 74 -- |g 6.3 |t Transposon-Mediated Approaches |g 78 -- |g 6.3.1 |t Transposon-Mediated Repositioning |g 78 -- |g 6.3.2 |t Transposon-Mediated Elimination |g 80 -- |g 6.4 |t Site-Specific Recombination |g 81 -- |g 6.4.1 |t The Cre/loxP System |g 82 -- |g 6.4.2 |t The FLP/frt System |g 86 -- |g 6.4.3 |t The R/RS System |g 88 -- |g 6.5 |t Intrachromosomal Homologous Recombination |g 89 -- |g 6.6 |t Conclusions and Future Prospects |g 90 -- |g 7 |t GST-MAT Vector for the Efficient and Practical Removal of Marker Genes from Transgenic Plants / |r H. Ebinuma, K. Sugita, E. Matsunaga, S. Endo, K. Yamada -- |g 7.2 |t ipt-Type MAT Vectors |g 96 -- |g 7.2.1 |t Transposable Element |g 96 -- |g 7.2.2 |t Site-Specific Recombination System |g 97 -- |g 7.2.3 |t Advantages of the ipt Gene |g 101 -- |g 7.3 |t Two-Step Transformation |g 103 -- |g 7.3.1 |t Promoter of the R Gene |g 103 -- |g 7.3.2 |t Promoter of the ipt Gene |g 105 -- |g 7.3.3 |t Combination of the ipt and iaaM/H Genes |g 108 -- |g 7.3.4 |t Transgene Stacking |g 110 -- |g 7.4 |t Single-Step Transformation |g 112 -- |g 7.5 |t Cloning Vector for Desired Genes |g 114 -- |g 8 |t Safety Assessment of Insect Protected Crops: Testing the Feeding Value of Bt Corn and Cotton Varieties in Poultry, Swine and Cattle / |r B. Hammond, E. Stanisiewski, R. Fuchs, J. Astwood, G. Hartnell -- |g 8.1.1 |t Food Safety Standards |g 119 -- |g 8.1.2 |t Testing for Food and Feed Safety |g 120 -- |g 8.2 |t Insect Protection Traits |g 122 -- |g 8.3 |t Benefits |g 123 -- |g 8.4 |t Safety Assessment of the Cry Insect-Control Proteins |g 124 -- |g 8.5 |t Mode of Action |g 125 -- |g 8.6 |t Substantial Equivalence Based on Compositional Analysis |g 126 -- |g 8.7 |t Current Products |g 127 -- |g 8.8 |t Grower Acceptance |g 127 -- |g 8.9 |t Future Products |g 127 -- |g 8.10 |t Farm-Animal Studies |g 128 -- |g 8.10.1 |t Bt Corn |g 128 -- |g 8.10.1.1 |t Poultry |g 128 -- |g 8.10.1.2 |t Lactating Cows |g 131 -- |g 8.10.1.3 |t Beef and Sheep |g 131 -- |g 8.10.1.4 |t Swine |g 132 -- |g 8.11 |t Cottonseed |g 133 -- |g 9 |t Safety Assessment of Genetically Modified Rice and Potatoes with Soybean Glycinin / |r K. Momma, W. Hashimoto, S. Utsumi, K. Murata -- |g 9.2 |t Safety Assessment of Genetically Modified Crops |g 140 -- |g 9.2.1 |t Genetically Modified Rice |g 141 -- |g 9.2.2 |t Genetically Modified Potatoes |g 144 -- |g 10 |t Chromosomal and Genetic Aberrations in Transgenic Soybean / |r R.J. Singh -- |g 10.2 |t Times in Culture with 2,4-D Prior to Transformation |g 154 -- |g 10.3 |t Genetic Background of the Explants |g 159 -- |g 10.4 |t Seed Fertility in Transgenic Soybean |g 160 -- |g 10.5 |t Cytological Basis of Gene Silencing |g 163 -- |g 11 |t Transgenic Barley (Hordeum vulgare L.) and Chromosomal Variation / |r M.-J. Cho, H.W. Choi, P. Bregitzer, S. Zhang, P.G. Lemaux -- |g 11.2 |t Chromosomal Variation in Nontransgenic Barley Plants |g 170 -- |g 11.3 |t Chromosomal Variation in Transgenic Barley Plants |g 172 -- |g 11.4 |t Fidelity and Quality of Transgenic Barley Plants |g 177 -- |g 11.4.1 |t Comparative Analysis of Genomic Stability in Plants Derived from Tissues Generated Using Different in Vitro Proliferation Processes |g 177 -- |g 11.4.2 |t Somaclonal Variation and Field Performance of Transgenic Plants Derived from Embryogenic Callus |g 179 -- |g 11.4.3 |t Stability of Transgenes and Transgene Expression |g 180 -- |g 11.5 |t Conclusions and Future Perspectives |g 184 |
520 | |a The debate over genetic manipulation and its use in plant improvement and protection has led to an increased demand for developing methods for detecting and characterizing genetic manipulation in plants and plant products such as seeds and foods. This book is unique in presenting all relevant methods together in one volume: those for using and determining markers retained in genetically manipulated products as well as methods for eliminating marker genes and procedures for characterizing chromosomal aberrations in genetically manipulated plants | ||
650 | 0 | |a Crops |x Genetic engineering | |
650 | 0 | |a Plant genetic engineering | |
650 | 0 | |a Plant genetic transformation | |
650 | 2 | |a Crops, Agricultural |x genetics | |
650 | 2 | |a Genetic Engineering | |
650 | 2 | |a Plants |x genetics | |
650 | 2 | |a Transformation, Genetic | |
650 | 7 | |a Crops |x Genetic engineering |2 fast | |
650 | 7 | |a Plant genetic engineering |2 fast | |
650 | 7 | |a Plant genetic transformation |2 fast | |
700 | 1 | |a Inman, R. B |q (Ross B.) |1 http://viaf.org/viaf/33610874 | |
700 | 1 | |a Inman, R. B |q (Ross B.) | |
700 | 1 | |a Jackson, J. F |q (John F.), |d 1935- | |
700 | 1 | |a Linskens, H. F |q (Hans F.), |d 1921- |1 http://viaf.org/viaf/66526639 | |
700 | 1 | |a Linskens, H. F |q (Hans F.), |d 1921- | |
700 | 1 | 0 | |a Jackson, J. F |q (John F.), |d 1935- |1 http://viaf.org/viaf/108295624 |
830 | 0 | |a Molecular methods of plant analysis ; |v v. 22 | |
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